人无柱式CD4⁺T细胞阳性分选试剂盒

人无柱式CD4⁺T细胞阳性分选试剂盒

产品名称：人无柱式CD4⁺T细胞阳性分选试剂盒

英文名称：Human CD4⁺ T Cell Positive Selection Kit (Column-Free)

产品货号：YK-NoColumn-003

靶细胞：人外周血PBMC/脐带血/白细胞富集液，无柱式磁珠阳性分选CD4⁺总T细胞

规格型号：

•YK-NoColumn-003（S）：富集1×10⁸个T细胞

•YK-NoColumn-003（M）：富集5×10⁸个T细胞

•YK-NoColumn-003（L）：富集1×10⁹个T细胞

分选原理：免疫磁珠阳性分选；抗人CD4抗体偶联超顺磁微珠特异性结合CD4⁺T细胞；无柱式强磁场直接捕获标记靶细胞，弃去未标记杂细胞；洗脱获得高纯度CD4⁺T细胞。

储存条件

• 生物素标记CD4+抗体：2~8℃避光保存，禁止冻融

• SA磁珠：2~8℃保存，切勿冷冻、干燥

• 缓冲液/洗涤液：室温密闭保存，避免污染

• 有效期：12个月

适用样本

• 人新鲜外周血单个核细胞（PBMC）

• 人脐带血单个核细胞

• 人骨髓/免疫细胞悬液（去除红细胞与死细胞后）

参考文献：

1.  EasySep™ Cell Separation Technology: A Simple, Column-Free Method for Isolating Highly Purified Cells. Technical Review, 2018.

2. Pezzi HM, Niles DJ. Integration of Magnetic Bead-Based Cell Selection into Complex Isolations. Cytometry Part A, 2004, 59: 191–202.

3. Richter A, Morrissey-Wettey FR, Angerer C. Column-free magnetic enrichment of pDCs yields significant contamination with DNA-containing dead cells that affect the activation status of the isolated cells. J Immunol, 2016, 196(Suppl 1): 209.20.

4. Fast and easy immunomagnetic positive selection of PE- or biotin-conjugated antibody-labelled cells with Releasable Rapidspheres results in highly purified, functional and particle-free cells. J Immunol (2017) 198(Suppl 1):81.10