小鼠无柱式NK细胞分选试剂盒
小鼠无柱式NK细胞分选试剂盒
产品名称:小鼠无柱式NK细胞阳性分选试剂盒
产品货号:YK-NoColumn-001m
靶细胞:小鼠外周血/单个核细胞中的pan NK细胞
规格型号:
•YK-NoColumn-001m(S):富集1×10⁸个NK细胞
•YK-NoColumn-001m(M):富集5×10⁸个NK细胞
•YK-NoColumn-001m(L):富集1×10⁹个NK细胞
产品原理
本试剂盒采用无柱式阳性分选策略,基于生物素-链霉亲和素(SA)高特异性结合原理实现NK细胞快速富集

储存条件
• 生物素标记CD56抗体:2~8℃避光保存,禁止冻融
• SA磁珠:2~8℃保存,切勿冷冻、干燥
• 缓冲液/洗涤液:室温密闭保存,避免污染
• 有效期:12个月
适用样本
• 小鼠新鲜外周血单个核细胞(PBMC)
• 小鼠骨髓/免疫细胞悬液(去除红细胞与死细胞后)
参考文献:
1. EasySep™ Cell Separation Technology: A Simple, Column-Free Method for Isolating Highly Purified Cells. Technical Review, 2018.
2. Pezzi HM, Niles DJ. Integration of Magnetic Bead-Based Cell Selection into Complex Isolations. Cytometry Part A, 2004, 59: 191–202.
3. Richter A, Morrissey-Wettey FR, Angerer C. Column-free magnetic enrichment of pDCs yields significant contamination with DNA-containing dead cells that affect the activation status of the isolated cells. J Immunol, 2016, 196(Suppl 1): 209.20.
4. Fast and easy immunomagnetic positive selection of PE- or biotin-conjugated antibody-labelled cells with Releasable Rapidspheres results in highly purified, functional and particle-free cells. J Immunol (2017) 198(Suppl 1):81.10


